antibodies against brd2  (Cell Signaling Technology Inc)

Journal: Pharmacological research

Article Title: YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer.

doi: 10.1016/j.phrs.2024.107283

Figure Lengend Snippet: Fig. 3. GAS41 collaborates with BRD2 and Mediator complex to regulate transcription of LMNB1, LMNB2, SYNE4 and LEMD2 genes. A Volcano plot of binding proteins interacting with GAS41 in the immunoprecipitation elute from HCT116 cells transfected with GFP and GFP-GAS41. The X axis represents log 2 Unique_Spectra_Num

Article Snippet: Bethyl Laboratories provided antibodies against BRD2 (A302–583A), BRD3 (A302–368A) and BRD4 (A301–985A100).

Techniques: Binding Assay, Immunoprecipitation, Transfection

Journal: Pharmacological research

Article Title: YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer.

doi: 10.1016/j.phrs.2024.107283

Figure Lengend Snippet: Fig. 4. Colocalization of GAS41 with BRD2 and H3K27ac throughout the genome. A Pie chart showing the distribution of Flag-GAS41 ChIP-seq peaks across the genome in HCT116 cells expressing Flag-GAS41. Promoter regions is defined as TSS ± 3 kb. B Venn diagram showing the overlap of Flag-GAS41, BRD2, H3K27ac and H3K27cr occupied genes in HCT116 cells expressing Flag-GAS41. C, D Average genome-wide profiles and heatmap profiles of Flag-GAS41, BRD2, H3K27ac and H3K27cr ChIP-seq peaks at TSSs ± 5 kb in HCT116 cells transiently expressing Flag-GAS41. E Genome browser views of the Flag-GAS41, BRD2, H3K27ac and H3K27cr ChIP-seq peaks on LMNB1 and LEMD2 in HCT116 cells expressing Flag-GAS41. F-H ChIP-qPCR analysis of BRD2 (F), MED14, MED23 (G) and RNA Pol II (H) occupancy on the indicated genes in HCT116 wt and GAS41 KO cells. The results in panels F, G and H are shown as mean + SEM from three biological replicates (two- tailed paired Student’s t test; *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant).

Article Snippet: Bethyl Laboratories provided antibodies against BRD2 (A302–583A), BRD3 (A302–368A) and BRD4 (A301–985A100).

Techniques: ChIP-sequencing, Expressing, Genome Wide, ChIP-qPCR, Two Tailed Test

Journal: Pharmacological research

Article Title: YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer.

doi: 10.1016/j.phrs.2024.107283

Figure Lengend Snippet: Fig. 5. GAS41 recruits BRD2 and Mediator complex to NE structural gene loci. A ChIP-qPCR analysis of BRD2 (left), MED14 (middle) and MED23 (right) occupancy on the indicated genes in HCT116 GAS41 KO cells stably expressing vector, Flag-GAS41 wt or Y74A W93A mutant. B ChIP-qPCR analysis of RNA polymerase II abundance on the indicated genes in HCT116 wt and GAS41 KO cells. C ChIP-qPCR analysis of H3K27ac abundance on the indicated genes in HCT116 wt, GAS41 KO (left) and GAS41 KO cells stably expressing vector, Flag-GAS41 wt or Y74A W93A mutant (right). D Proposed mechanisms of GAS41 mediated regulation of nuclear shape. The results in panels A-C are shown as mean + SEM from three biological replicates (two-tailed paired Student’s t test; *P < 0.05, **P < 0.01; ***P < 0.001; ns, not significant).

Article Snippet: Bethyl Laboratories provided antibodies against BRD2 (A302–583A), BRD3 (A302–368A) and BRD4 (A301–985A100).

Techniques: ChIP-qPCR, Stable Transfection, Expressing, Plasmid Preparation, Mutagenesis, Two Tailed Test

Journal: Cell reports

Article Title: HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies

doi: 10.1016/j.celrep.2020.108638

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Lysates were boiled with SDS loading buffer; equal volumes of ‘soluble’ and ‘chromatin’ fractions were loaded onto an SDS-PAGE gel and western blot analyses were performed using antibodies against BRD2 (Bethyl A302–583A), BRD3 (Bethyl A302–368A), BRD4 (Bethyl A301–985A100), CDK9 (ThermoFisher 703404).

Techniques: Recombinant, Staining, Protease Inhibitor, SYBR Green Assay, DNA Purification, Western Blot, Software